Journal: Journal of Cancer

Article Title: Ophiopogonin B alleviates cisplatin resistance of lung cancer cells by inducing Caspase-1/GSDMD dependent pyroptosis

doi: 10.7150/jca.66432

Figure Lengend Snippet: OP-B also had a significant inhibitory effect on A549/DDP carcinoma in situ in mice and transplanted tumors in zebrafish. A. Bioluminescent imaging and quantification of photon flux of 3 mg/kg OP-B treated groups with right lung parenchyma injection of luciferase-marked A549 and A549/DDP cells. B. Before and after OP-B treatment, the fluorescence intensity changes on model and OP-B groups of A549 and A549/DDP. C. Tumor growth rate of A549 (a) and A549/DDP (b) calculated according to the fluorescence intensity data. D. Comparison of tumor growth rates between A549 and A549/DDP. E. Representative images taken by fluorescence stereomicroscope of the zebra fish that were injected with A549 and A549/DDP cells and treated with 5 μM OP-B for 3 days. F. Relative fluorescence intensity of A549 (a) and A549/DDP (b) tumor in zebra fish after OP-B treatment. G. Comparison of tumor growth rate between A549 and A549/DDP that calculated according to the fluorescence intensity data. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001

Article Snippet: At 72 h post injection, the proliferation of tumor cells was evaluated through a fluorescence stereomicroscope (OLYMPUS U-HGLGPSD, equipped with Cell Sens Entry software, Tokyo, Japan).

Techniques: In Situ, Imaging, Injection, Luciferase, Fluorescence, Comparison

Journal: PLOS One

Article Title: A single PCR-sequencing method to establish the frequency of kdr alleles in the stable fly, Stomoxys calcitrans : application to seven livestock farms from south of France

doi: 10.1371/journal.pone.0332229

Figure Lengend Snippet: Hybridization sites of the primers F0994 and R1053 are underlined in bold; nucleotides of the codon 1014 are in bold with * (the sequence CTT of the wild fly, is coding for Leucine); in italics are the nucleic acid of the intron (other nucleic acids, in blocs of 3 letters, are codons of the sodium channel protein).

Article Snippet: After amplifying part of the Vssc domain II, the PCR products and the primer sequences were sent for double sense sequencing (Sanger sequencing) at the pharmaceutical company Eurofins CDMO (Contract Development & Manufacturing Organization).

Techniques: Hybridization, Sequencing

Journal: PLOS One

Article Title: A single PCR-sequencing method to establish the frequency of kdr alleles in the stable fly, Stomoxys calcitrans : application to seven livestock farms from south of France

doi: 10.1371/journal.pone.0332229

Figure Lengend Snippet: Each nucleotide is represented by a colour: Cytosine/blue, Thymine/red and Adenine/green; peaks of the chromatogram curves indicate the type of nucleotide present at this location. The 3 lines below the chromatograms are from the top to the bottom: First line (coloured block letters): sequencing results indicated by the sequencing machine, based on the highest peaks. Second line (coloured block letters): sequencing results indicated by the secondary peak (heterozygoty) when present. Third line (black block-letters): sequence (homozygoty) or coded sequences (heterozygoty) obtained. For example, in the second codon from left: the first line indicates dominant nucleotides CTT (the red line, for Thymidine, is the highest in the 2 last locations), the second line indicates CAT (the green line is almost equally high in the second location); therefore, this codon reveals heterozygoty and the second nucleotide, T or A, is coded as “W”, in black colour, on the third line, showing “CWT”. W means A or T; Y means C or T.

Article Snippet: After amplifying part of the Vssc domain II, the PCR products and the primer sequences were sent for double sense sequencing (Sanger sequencing) at the pharmaceutical company Eurofins CDMO (Contract Development & Manufacturing Organization).

Techniques: Blocking Assay, Sequencing